Fig. 3. (A) Flag-NEDD4 was expressed in 293T cells, and Myc-YOD1 was transfected in a dose-dependent manner. (B) Using mouse samples, Western blotting was performed to analyze the expression levels of YOD1, NEDD4, and p-YAP. (C) Using mouse samples, Western blotting was performed to analyze the expression levels of NEDD4. (D) The cell seeding density was 100 cells, grown in 100-mm dishes for 14 days, and stained with crystal violet. (E) 5,000 cells were transferred into 96-well plates. After 12 hrs, the cells were incubated with a medium containing CCK-8 for 4 hrs and OD was measured at 450 nm.